sequencing libraries by tagmentation nexteraxt Search Results


nexteraxt tagmentase  (Illumina Inc)
97
Illumina Inc nexteraxt tagmentase
(A) Genomic DNA is isolated from the organism/tissue of interest. Tissue is homogenized and treated with SDS and proteinase K. To enrich for circular DNAs, total genomic DNA (G) is treated with exoV (Palas and Kushner) to produce G exo or banded in a CsCl gradient to separate G into GT and GB.(Grossman et al.) GT is the upper band of the gradient and includes linear DNAs and nicked circular DNAs. GB, the bottom band, consists of covalently closed-circular DNAs. After enrichment for circular DNA with either method (or both), eccDNA is minimally sheared by attenuated treatment with Nextera <t>tagmentase.</t> (B) Transposition creates a 9-bp sequence duplication flanking the transposon insertion site. Tn5 randomly binds and cuts DNA, leaving a staggered, 9-nucleotide single-stranded overhang. DNA on either side of the cut is filled by DNA polymerase in the first PCR cycle, thereby creating 9-bp duplications flanking the genomic DNA sequence. Matching overhangs in the figure have matching colors. Also, paired reads (R1 and R2, indicated by arrows) share the same color. If a circular DNA molecule gets cut only once by Tn5, paired-end sequencing will reveal a unique 9-bp duplication at the beginning of each read (designated by colored overhangs); thereby providing a bioinformatic mark for circular DNAs.
Nexteraxt Tagmentase, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing+libraries+by+tagmentation+nexteraxt/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/bio_rxiv__128686-49-19-21
Average 97 stars, based on 1 article reviews
nexteraxt tagmentase - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier
nexteraxt dna library preparation kit  (Illumina Inc)
99
Illumina Inc nexteraxt dna library preparation kit
(A) Genomic DNA is isolated from the organism/tissue of interest. Tissue is homogenized and treated with SDS and proteinase K. To enrich for circular DNAs, total genomic DNA (G) is treated with exoV (Palas and Kushner) to produce G exo or banded in a CsCl gradient to separate G into GT and GB.(Grossman et al.) GT is the upper band of the gradient and includes linear DNAs and nicked circular DNAs. GB, the bottom band, consists of covalently closed-circular DNAs. After enrichment for circular DNA with either method (or both), eccDNA is minimally sheared by attenuated treatment with Nextera <t>tagmentase.</t> (B) Transposition creates a 9-bp sequence duplication flanking the transposon insertion site. Tn5 randomly binds and cuts DNA, leaving a staggered, 9-nucleotide single-stranded overhang. DNA on either side of the cut is filled by DNA polymerase in the first PCR cycle, thereby creating 9-bp duplications flanking the genomic DNA sequence. Matching overhangs in the figure have matching colors. Also, paired reads (R1 and R2, indicated by arrows) share the same color. If a circular DNA molecule gets cut only once by Tn5, paired-end sequencing will reveal a unique 9-bp duplication at the beginning of each read (designated by colored overhangs); thereby providing a bioinformatic mark for circular DNAs.
Nexteraxt Dna Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing+libraries+by+tagmentation+nexteraxt/Nextera+XT+DNA+Library+Preparation+Kit/pm36084631-374-16-15
Average 99 stars, based on 1 article reviews
nexteraxt dna library preparation kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


(A) Genomic DNA is isolated from the organism/tissue of interest. Tissue is homogenized and treated with SDS and proteinase K. To enrich for circular DNAs, total genomic DNA (G) is treated with exoV (Palas and Kushner) to produce G exo or banded in a CsCl gradient to separate G into GT and GB.(Grossman et al.) GT is the upper band of the gradient and includes linear DNAs and nicked circular DNAs. GB, the bottom band, consists of covalently closed-circular DNAs. After enrichment for circular DNA with either method (or both), eccDNA is minimally sheared by attenuated treatment with Nextera tagmentase. (B) Transposition creates a 9-bp sequence duplication flanking the transposon insertion site. Tn5 randomly binds and cuts DNA, leaving a staggered, 9-nucleotide single-stranded overhang. DNA on either side of the cut is filled by DNA polymerase in the first PCR cycle, thereby creating 9-bp duplications flanking the genomic DNA sequence. Matching overhangs in the figure have matching colors. Also, paired reads (R1 and R2, indicated by arrows) share the same color. If a circular DNA molecule gets cut only once by Tn5, paired-end sequencing will reveal a unique 9-bp duplication at the beginning of each read (designated by colored overhangs); thereby providing a bioinformatic mark for circular DNAs.

Journal: bioRxiv

Article Title: Beyond the Linear Genome: Comprehensive Determination of the Endogenous Circular Elements in C. elegans and Human Genomes via an Unbiased Genomic-Biophysical Method

doi: 10.1101/128686

Figure Lengend Snippet: (A) Genomic DNA is isolated from the organism/tissue of interest. Tissue is homogenized and treated with SDS and proteinase K. To enrich for circular DNAs, total genomic DNA (G) is treated with exoV (Palas and Kushner) to produce G exo or banded in a CsCl gradient to separate G into GT and GB.(Grossman et al.) GT is the upper band of the gradient and includes linear DNAs and nicked circular DNAs. GB, the bottom band, consists of covalently closed-circular DNAs. After enrichment for circular DNA with either method (or both), eccDNA is minimally sheared by attenuated treatment with Nextera tagmentase. (B) Transposition creates a 9-bp sequence duplication flanking the transposon insertion site. Tn5 randomly binds and cuts DNA, leaving a staggered, 9-nucleotide single-stranded overhang. DNA on either side of the cut is filled by DNA polymerase in the first PCR cycle, thereby creating 9-bp duplications flanking the genomic DNA sequence. Matching overhangs in the figure have matching colors. Also, paired reads (R1 and R2, indicated by arrows) share the same color. If a circular DNA molecule gets cut only once by Tn5, paired-end sequencing will reveal a unique 9-bp duplication at the beginning of each read (designated by colored overhangs); thereby providing a bioinformatic mark for circular DNAs.

Article Snippet: To generate fragmented genomic DNA libraries with appropriate linkers, 1 ng of DNA was treated with 1.5 ul of NexteraXT tagmentase (illumina) at 37 °C for 30 min with gentle shaking.

Techniques: Isolation, Sequencing

A 3-kbp plasmid DNA (derived from pGEM5Zf+) and 412-bp circular DNA molecules were fragmented using Nextera-XT tagmentase. This graph provides a position-by-position summary of aligned reads. The orange arrow indicates a read length of a singly-tagmented circle, 421 bp (412 bp + 9 bp duplication)

Journal: bioRxiv

Article Title: Beyond the Linear Genome: Comprehensive Determination of the Endogenous Circular Elements in C. elegans and Human Genomes via an Unbiased Genomic-Biophysical Method

doi: 10.1101/128686

Figure Lengend Snippet: A 3-kbp plasmid DNA (derived from pGEM5Zf+) and 412-bp circular DNA molecules were fragmented using Nextera-XT tagmentase. This graph provides a position-by-position summary of aligned reads. The orange arrow indicates a read length of a singly-tagmented circle, 421 bp (412 bp + 9 bp duplication)

Article Snippet: To generate fragmented genomic DNA libraries with appropriate linkers, 1 ng of DNA was treated with 1.5 ul of NexteraXT tagmentase (illumina) at 37 °C for 30 min with gentle shaking.

Techniques: Plasmid Preparation, Derivative Assay